引用本文: 唐仕娟，苗金贝，陈艳琳，邵娅，路康，王森山. 齐墩果酸对豌豆蚜生长发育及繁殖的影响. 草业科学, 2019, 36(10): 2666-2673. doi: shu

Citation: TANG S J, MIAO J B, CHENG Y L, SHAO Y, LU K, WANG S S. Effect of oleanolic acid on the growth and reproduction of the pea aphid. Pratacultural Science, 2019, 36(10): 2666-2673. doi: shu

齐墩果酸对豌豆蚜生长发育及繁殖的影响

.
BG视讯 gansunongyedaxuezhiwubaohuxueyuan / gansushengnongzuowubingchonghaishengwufangzhigongchengshiyanshi，gansu lanzhou 730070

作者简介: 唐仕娟(1995-)，女，甘肃永登人，在读研究生，研究方向为植物病理学。E-mail: ;

通讯作者: 王森山, .cn

基金项目: 甘肃省高等学校科研项目(2016B-040)；甘肃农业大学大学生SRTP项目(20171115)

摘要: 齐墩果酸是一种三萜类化合物，对植食性昆虫具有拒食、毒杀、趋避作用。为明确齐墩果酸对两种色型豌豆蚜生理的影响，本研究采用豌豆蚜全纯人工饲料薄膜饲养技术。通过设置5组不同浓度的齐墩果酸人工饲料，在室内培养箱环境下饲养绿色型、红色型豌豆蚜(Acyrthosiphon pisum)，测定了存活率、相对日均体重增长率、有翅蚜率和产蚜率等指标。结果表明，齐墩果酸对绿色型豌豆蚜的生长发育及繁殖具有一定的抑制作用，而对红色型豌豆蚜的生长发育及繁殖没有抑制作用。在齐墩果酸浓度0.1～2.0 mg·mL^–1内，随着齐墩果酸浓度的增大，绿色型豌豆蚜的存活率、相对日均体重增长率、平均产蚜量、产蚜率均表现为降低，而在2.0 mg·mL^–1时降至最低。而有翅蚜率逐渐增大并在1.0 mg·mL^–1浓度时达到最大，随后逐渐降低。本研究为进一步研究齐墩果酸在生理、行为学层面是如何影响豌豆蚜的生长发育等提供技术和依据。

关键词:

齐墩果酸 /
豌豆蚜 /
人工饲料 /
繁殖

English

Effect of oleanolic acid on the growth and reproduction of the pea aphid

college of plant protection, gansu agricultural university / biocontrol engineering laboratory of crop diseases and pests of gansu province, lanzhou 730070, gansu, china

Corresponding author: Senshan WANG, .cn

Received Date: 2018-12-27
Available Online: 2019-10-01

Abstract: Oleanolic acid, a triterpenoid, has antifeedant, lethal, and avoidance effects on herbivorous insects. This study aimed to clarify the effects of oleanolic acid on the physiology of two color morphs of pea aphid (Acyrthosiphon pisum). For this, the pure artificial diet film feeding technology was applied and 5 groups of artificial diet, with different concentrations of oleanolic acid, were prepared to rear the green and red morph pea aphids in incubators. The following parameters were measured: survival rate, growth rate of relative daily average weight, ratio of winged phenotype, and percentage of reproducing individuals. The results showed that oleanolic acid had inhibitory effects on the growth and reproduction of the green morph, but did not affect the red morph. In the range of 0.1～2.0 mg·mL^–1, all the measured parameters (except the ratio of winged phenotype) of the green morph decreased with the increase of the oleanolic acid concentration, and the lowest data was obtained at 2.0 mg·mL^–1. The ratio of winged phenotype gradually increased with the increase of the oleanolic acid concentration and reached a maximum at the concentration of 1.0 mg·mL^–1, but then gradually decreased. These results will lay a technological and theoretical foundation for further studies on the physiological and behavioral mechanisms of how oleanolic acid affects the development and reproduction of the pea aphid.

Key words:

1. [1]
  匡海学.中药化学.北京:中国中医药出版社, 2003:226-259.
  BG视讯 KUANG H X. Traditional Chinese Medicine Chemistry. Beijing: Chinese Traditional Medicine Press, 2003:226-259.
2. [2]
  孙彦, 龙瑞才, 张铁军, 杨青川, 周禾. 紫花苜蓿皂苷研究进展[J]. 草业学报草业学报, 2013, 22(3): 274-283.
  SUN Y, LONG R C, ZHANG T J, YANG Q C, ZHOU H. Research progress on saponins of alfalfa[J]. Journal of Grass IndustryJournal of Grass IndustryBG视讯, 2013, 22(3): 274-283.
3. [3]
  彭少麟, 南蓬, 钟扬. 植物中的萜类化合物及其在生态系统中的作用[J]. 生态学杂志生态学杂志, 2002, (3): 33-38. doi:
  PENG S L, NAN P, ZHONG Y. Indole compounds in higher plants and their roles in ecosystems[J]. Journal of EcologyJournal of Ecology, 2002, (3): 33-38. doi:
4. [4]
  景沛, 鲁庭延, 熊丹, 李劲薇, 刘易陇, 钟志容. 齐墩果酸片的处方优化设计[J]. 泸州医学院学报泸州医学院学报, 2014, 37(3): 235-238. doi:
  JIANG P, LU T Y, XIONG D, LI J W, LIU Y L, ZHONG Z R. Prescription optimization design of oleanolic acid tablets[J]. Journal of Luzhou Medical CollegeJournal of Luzhou Medical College, 2014, 37(3): 235-238. doi:
5. [5]
  张亮.新疆紫花苜蓿皂苷的提取、分离及纯化工艺研究.乌鲁木齐: 新疆大学硕士学位论文, 2009.
  ZHANG L. Study on the saponins of extraction, separation and purification of Xinjiang alfalfa. Master Thesis. Urumqi: Xinjiang University, 2009.
6. [6]
  付佳, 王洋, 阎秀峰. 萜类化合物的生理生态功能及经济价值[J]. 东北林业大学学报东北林业大学学报, 2003, 31(6): 59-62. doi:
  FU J, WANG Y, PEI X F. Economic of physiological and ecological functions value of terpenoids[J]. Journal of Northeast Forestry University, 2003, 31(6): 59-62. doi:
7. [7]
  徐正浩, 崔绍荣, 何勇, 李迪, 赵明, 张旭, 余柳青. 植物次生代谢物质和害虫防治[J]. 植物保护植物保护, 2004, 30(4): 8-11. doi:
  XU Z H, CUI S R, HE Y, LI D, ZHAO M, ZHANG X, YU L Q. Plant secondary metabolites and pest control[J]. Plant ProtectionPlant Protection, 2004, 30(4): 8-11. doi:
8. [8]
  GOVINDACHARI T R, SURESH G. Antifungal activity of some B, D-secolimoniods from two Meliaceous plants[J]. Journal of Chemical EcologyJournal of Chemical Ecology, 1999, 25(4): 923-933. doi:
9. [9]
  谷文祥, 段舜山, 骆世明. 萜类化合物的生态特性及其对植物的化感作用[J]. 华南农业大学学报华南农业大学学报, 1998, 19(4): 111-115.
  GU W X, DUAN Y S, LUO S M. The ecological characteristics of indole compounds and their allelopathic effects on plants[J]. Journal of South China Agricultural UniversityBG视讯, 1998, 19(4): 111-115.
10. [10]
  董红霞, 王敬淑, 刘光华, 凌冰. 植物次生化合物在害虫防治中的作用[J]. 仲恺农业技术学院学报仲恺农业技术学院学报, 2005, 18(2): 65-71. doi:
  DONG H X, WANG J S, LIU G H, LING B. The roles of secondary metabolites in insect pest control[J]. Journal of Zhongkai University of Agriculture and Technolog, 2005, 18(2): 65-71. doi:
11. [11]
  吕宁, 刘长仲. 不同抗生素对豌豆蚜生物学特性的影响[J]. 中国生态农业学报中国生态农业学报, 2014, 22(2): 208-216.
  LYU N, LIU C Z. Effect of different antibiotics on the biological characteristics of pea aphid[J]. Chinese Journal of Eco-AgricultureChinese Journal of Eco-Agriculture, 2014, 22(2): 208-216.
12. [12]
  朱玉永. 豌豆蚜与寄主互作关系的研究. 石河子: 石河子大学硕士学位论文, 2014.
  BG视讯 ZHU Y Y. Study on the interaction between aphids and their host. Master Thesis. Shihezi: Shihezi University, 2014.
13. [13]
  武德功, 杜军利, 贺春贵. 4个苜蓿品种对两种体色豌豆蚜的抗生性[J]. 植物保护植物保护, 2015, 41(): 49-54, 62. doi:
  WU D G, DU J L, HE C G. Antibiosis of four alfalfa cultivars against two color morphs of Acyrthosiphon pisum[J]. Plant ProtectionPlant Protection, 2015, 41(): 49-54, 62. doi:
14. [14]
  武德功, 王森山, 刘长仲, 胡桂馨, 杜军利, 贺春贵. 豌豆蚜刺吸胁迫对不同苜蓿品种体内单宁含量及生理活性的影响[J]. 草地学报草地学报, 2011, 19(): 351-355. doi:
  WU D G, WANG S S, LIU C Z, HU G X, DU J L, HE C G. Effects of herbivore stress by Acyrthosiphon pisum on the contents of Tannin and physiological activity in different alfalfa cultivars[J]. Acta Agrestia SinicaActa Agrestia Sinica, 2011, 19(): 351-355. doi:
15. [15]
  孙玺文. 光照与温度交互作用对两种色型豌豆蚜种群的影响.兰州: 甘肃农业大学硕士学位论文, 2017.
  SUN X W. Effects of photo period and temperature interaction on population of two colour morphs of Acyrthosiphon pisumBG视讯. Master Thesis. Lanzhou: Gansu Agricultural University, 2017.
16. [16]
  BRAENDLE C, WEISSER W W. Variation in escape behavior of red and green clones of the pea aphid[J]. Journal of Insect BehaviorJournal of Insect Behavior, 2001, 14(4): 497-509. doi:
17. [17]
  FARHOUDI F, ALLAHYARI H, TABADKANI S M, GHOLIZADEH M. Prey preference of Aphidoletes aphidimyza on Acyrthosiphon pisum: Effect of prey color and size[J]. Journal of insect BehaviorJournal of insect Behavior, 2014, 27(6): 776-785. doi:
18. [18]
  LOSEY J E, HARMON J, BALLANTYNE F, BROWN C. A polymorphism maintained by opposite patterns of parasitism and predation[J]. NatureNature, 1997, 388(): 269-272. doi:
19. [19]
  FRANTZ A, CALCAGNO V, MIEUZET L, PLANTEGENEST M, SIMON J C. Complex trait differentiation between host- populations of the pea aphid Acyrthosphon pisum (Harris): Implications for the evolution of ecological specialisation[J]. Biological Journal of the Lin-nean Socicty, 2009, 97(4): 718-727. doi:
20. [20]
  LIBBRECHT R, GWYNN D M, FELLOWES M D E. Aphidius ervi preferentially attacks the green morph of the pea aphid, Acyrthosiphon pisum[J]. Journal of insect BehaviorJournal of insect Behavior, 2007, 20(1): 25-32. doi:
21. [21]
  王小强, 刘长仲, 祁发鹏, 李毅恒. 吡虫啉亚致死剂量对2种色型豌豆蚜生长发育和种群参数的影响[J]. 草地学报草地学报, 2014, 22(5): 1110-1116. doi:
  WANG X Q, LIU C Z, QI F P, LI Y H. Effects of sublethal dosage of imidacloprid on the growth, development and population parameter of two color morphs of pea aphid[J]. Acta Agrectia SinicaActa Agrectia Sinica, 2014, 22(5): 1110-1116. doi:
22. [22]
  AHSAEI S M, TABADKANI S M, HOSSEININAVEH V, ALLAHYARI H, BIGHAM M. Differential accumulation of energy by the colour morphs of the pea aphid Acyrthosiphon pisum mirrors their ecological adaptations[J]. European Journal of EntomologyEuropean Journal of Entomology, 2013, 110(2): 241-245. doi:
23. [23]
  SCHUETT W, DALL S R, KLOESENER M H, BAEUMER J, BEINLICH F, EGGERS T. Life-history trade-offs mediate‘personality’variation in two colour morphs of the pea aphid, Acyrthosiphon pisum[J]. Journal of Animal EcologyJournal of Animal Ecology, 2015, 84(1): 90-101. doi:
24. [24]
  王小强, 刘长仲. 阿维菌素亚致死剂量下2种色型豌豆蚜解毒酶活力的研究[J]. 中国生态农业学报中国生态农业学报, 2014, 22(6): 675-681.
  WANG X Q, LIU C Z. Detoxification enzymes activities in two color morphs of pea aphid (Acyrthosiphon pisum) treated with different sub-lethal concentrations of avermectin[J]. Chinese Journal of Eco-AgricultureChinese Journal of Eco-AgricultureBG视讯, 2014, 22(6): 675-681.
25. [25]
  LI Z, WAGN M Y, LI X P, WANG X T, JIA C L, YANG X Z, FENG R Q, YUAN M L. A small set of differentially expressed genes was associated with two color morphs in natural populations of the pea aphid Acyrthosiphon pisum[J]. GeneGene, 2018, 651(): 23-32.
26. [26]
  邵娅, 王森山, 叶超. 单宁酸对红、绿色型豌豆蚜生长发育及繁殖的影响[J]. 草地学报草地学报, 2017, 25(): 866-870. doi:
  SHAO Y, WANG S S, YE C. Effects of tannic acid on growth and reproduction of red and green aphids[J]. Acta Grassland SinicaActa Grassland Sinica, 2017, 25(): 866-870. doi:
27. [27]
  叶超. 豌豆蚜人工饲料及饲养技术研究. 兰州: 甘肃农业大学硕士学位论文, 2016.
  YE C.Investigation on artificial diet and rearing technique of pea aphid (Acyrthosiphon pisum Harris). Master Thesis. Lanzhou: Gansu Agricultural University, 2016.
28. [28]
  POLLARD D. Plant penetration by feeding aphids (Hemiptera, Aphidoidea): A review[J]. Bulletin of Entomological ResearchBulletin of Entomological Research, 1973, 62(): 631-714. doi:
29. [29]
  TJALLINGⅡ W, ESCH T H. Fine structure of aphid stylet routes in plant tissues in correlation with EPG signals[J]. Physiological EntomologyPhysiological Entomology, 1993, 18(): 317-328. doi:
30. [30]
  KANG J H, LIU G, SHI F, JONES A, BEAUDRY R M, HOWE G A. The tomatoodorless-2 mutant is defective in trichome-based production of diverse specializedmetabolites and broad-spectrum resistance to insect herbivores[J]. Plant PhysiologicalPlant Physiological, 2010, 154(): 262-272. doi:
31. [31]
  GRIFFITHS D W, DEIGHTON N, BIRCH A N E, PATRIAN B, BAUR R, STÄDLER E. Identification of glucosinolates on the leaf surface of plants from the cruciferaeand other closely related species[J]. PhytochemistryPhytochemistry, 2001, 57(): 693-700. doi:
32. [32]
  REINA-PINTO J J, YEPHREMOV A. Surface lipids and plant defenses[J]. Plant Physiological BiochemistryPlant Physiological Biochemistry, 2009, 47(): 540-549. doi:
33. [33]
  GLAS J J, SCHIMMEL B C, ALBA J M, ESCOBAR-BRAVO R, SCHUURINK R C, KANT M R. Plant glandular trichomes as targets for breeding or engineering of resistance toherbivores[J]. International Journal of Molecular Sciences, 2012, 13(): 17077-17103. doi:
34. [34]
  杨巧燕. 豌豆蚜体内共生菌对蚜虫与寄主互作关系的影响研究. 兰州: 甘肃农业大学硕士学位论文, 2017.
  YANG Q Y. Research on the effects of the intracellular bacterial symbionts in Acyrthosiphon pisum harris on the interaction between aphids and host plants. Master Thesis. Lanzhou: Gansu Agricultural University, 2017.
35. [35]
  KOGA R, TSUCHIDA T, FUKATSU T. Changing partners in an obligate symbiosis: A facultative endosymbiont can compensate for loss of the essential endosymbiont Buchnera in an aphid[J]. Proceedings of the Royal Society B:Biological Sciences, 2003, 270(): 2543-2550. doi:
36. [36]
  刘向东, 张元臣. 蚜虫共生菌感染格局、动态及在宿主种群分化中的作用[J]. 南京农业大学学报南京农业大学学报, 2018, 41(2): 209-217. doi:
  LIU X D, ZHANG Y C. Infection pattern and dynamics of endosymbionts in aphids and their effects on population differentiation of hosts[J]. Journal of Nanjing Agricultural University, 2018, 41(2): 209-217. doi:
37. [37]
  王宪楷. 天然药物化学. 北京: 人民卫生出版社, 1988: 391-460.
  BG视讯 WANG X K. Natural Pharmaceutical Chemistry. Beijing: People's Medical Publishing House, 1988: 391-460.
38. [38]
  张亚妮.植物源杀虫剂川楝素环境安全性评价.杨凌: 西北农林科技大学硕士学位论文, 2007.
  ZHANG Y N. Environmental safety evaluation of botanical pesticide toosedanim. Master Thesis. Yangling: Northwest A & F University.2007.
39. [39]
  汪文陆, 赵善欢, 韩玖, 徐应生. 苦楝中几种杀虫有效成分对菜青虫和亚洲玉米螟的生物活性[J]. 植物保护学报植物保护学报, 1992, (4): 359-364.
  WANG W L, ZHAO S X, HAN J, XU Y S. Bioactivity of several insecticidal active components in neem against cabbage and Asiatic corn borer[J]. Journal of Plant ProtectionJournal of Plant ProtectionBG视讯, 1992, (4): 359-364.
40. [40]
  胡江川, 孙爱芹, 路明花. 苦楝皮活性成分对几种地下害虫的杀虫效果研究[J]. 北方园艺北方园艺, 2012, (7): 147-149.
  HU J C, SONG A Q, LU M H. Study on the insecticidal effect of neem bark active components on several underground pests[J]. Northern HorticultureNorthern HorticultureBG视讯, 2012, (7): 147-149.
41. [41]
  董易之, 张茂新, 凌冰. 葫芦素B对甜菜夜蛾幼虫取食和成虫产卵的影响[J]. 华南农业大学学报华南农业大学学报, 2005, 26(2): 56-58. doi:
  DONG Y Z, ZhANG M X, LING B. Influence of cucurbitacin B on feeding behavior and oviposition of Spodoptera exigua[J]. Journal of South China Agricultural University, 2005, 26(2): 56-58. doi:
42. [42]
  张茂新, 凌冰. 六种植物叶片中葫芦素B对美洲斑潜蝇寄主选择性的影响[J]. 生态学报生态学报, 2004, 24(11): 2564-2568. doi:
  ZHANG M X, LING B. Effect of cucurbitacin B in leaves from six plants species on plant selectivity of the leaf miner, Liriomyza sativae[J]. Acta EcologicasinicaActa Ecologicasinica, 2004, 24(11): 2564-2568. doi:
43. [43]
  王树栋. 四种植物源化合物对甜菜夜蛾酚氧化酶的影响及酶免疫学研究. 泰安: 山东农业大学硕士学位论文, 2010.
  WANG S D.Study on the effects of four plant-derived compounds on phenoloxidase of Spodotera exiguaBG视讯 (Hübner) and immunology of the enzyme. Master Thesis. Tai'an: Shandong Agricultural University, 2010.
1. [1]
  张洪英 , 魏淑花 , 张蓉 , 苗润 , 李克昌 , 罗晓玲 , 张宇 . 豌豆蚜为害对苜蓿品种酶活性和营养物质的影响. 草业科学, 2016, 10(1): 144-152. doi: BG视讯
2. [2]
  马亚玲 , 刘长仲 , 李春杰 . 光周期和强度对豌豆蚜子代生物学特性的影响. 草业科学, 2019, 36(12): 3182-3190. doi: BG视讯
3. [3]
  杨巧燕 , 陈威 , 孙小玲 , 刘长仲 . 多异瓢虫捕食豌豆蚜功能对3种杀虫剂亚致死剂量的响应. 草业科学, 2016, 10(7): 1418-1425. doi:
4. [4]
  王玉祥 , 张博 . 新疆野生三叶草弹花机制及繁殖特性. 草业科学, 2016, 10(4): 662-668. doi:
5. [5]
  钱瑭璜 , 雷江丽 , 许建新 , 徐义炎 . 华南地区8种地被植物的耐旱性及繁殖移栽效果. 草业科学, 2013, 7(11): 1718-1724.
6. [6]
  豌豆蚜生物生态学特性. 草业科学, 2017, 11(8): 1727-1740. doi:
7. [7]
  4gemuxupinzhongduiwandouyadekangxingpingjia. caoyekexue, 2012, 6(1): 101-104.
8. [8]
  杜军利 , 武德功 , 黄保宏 , 黄伟东 , 王增霞 . 异色瓢虫对豌豆修尾蚜的捕食功能反应. 草业科学, 2018, 12(7): 1772-1779. doi: BG视讯
9. [9]
  魏淑花 , 曹春婧 , 朱小芳 , 张蓉 , 王颖 . 豌豆蚜在6个不同抗性苜蓿品种上取食行为的EPG分析. 草业科学, 2017, 11(12): 2569-2574. doi:
10. [10]
  fengyutiaojianxiabaimaofanzhiduiceyanjiu. caoyekexue, 2008, 2(11): 79-83.
11. [11]
  李伟丽 , 黄丽芳 , 夏新界 . 芡欧鼠尾草的组织培养和快速繁殖. 草业科学, 2016, 10(3): 393-399. doi: BG视讯
12. [12]
  mucaozhongzhiziyuanfanzhigengxinjishuguicheng. caoyekexue, 2009, 3(10): 134-139.
13. [13]
  王业社 , 段林东 , 周双 . 粗叶悬钩子无性扦插繁殖技术的优化. 草业科学, 2014, 8(8): 1461-1465. doi:
14. [14]
  周延山 , 花立民 , 纪维红 , 楚彬 , 刘丽 . 鼢鼠亚科繁殖特性研究进展. 草业科学, 2015, 9(6): 1010-. doi: BG视讯
15. [15]
  姚峰 , 蔺银鼎 . 绢毛匍匐委陵菜的适宜营养繁殖方式. 草业科学, 2016, 10(4): 700-705. doi:
16. [16]
  王倩 , 贾婷婷 , 庞晓攀 , 金少红 , 陈传武 , 郭正刚 . 高原鼠兔干扰对达乌里秦艽繁殖特征的影响. 草业科学, 2015, 9(5): 796-. doi:
17. [17]
  BG视讯 hudielanyounenhuagengzuzhipeiyanghekuaisufanzhi. caoyekexue, 2011, 5(4): 590-596.
18. [18]
  高欢欢 , 曾凡江 , 李尝君 , 郭自春 . 疏叶骆驼刺根系的田间离体繁殖. 草业科学, 2016, 10(5): 891-896. doi: BG视讯
19. [19]
  杨艳莉 , 周青平 , 颜红波 , 石红霄 . 行距对青海扁茎早熟禾无性繁殖影响的研究. 草业科学, 2009, 3(5): 66-71.
20. [20]
  刘晓东 , 刘娇 , 何淼 . 香蓼带芽茎段的组织培养和快速繁殖. 草业科学, 2014, 8(1): 89-94. doi: BG视讯

BG视讯

图 1 BG视讯不同浓度齐墩果酸对绿色型豌豆蚜产蚜量的影响

Figure 1. Effect of different concentrations oleanolic acid on green pea aphid fecundity

下载: 全尺寸图片幻灯片

图 2 不同浓度齐墩果酸对红色型豌豆蚜平均产蚜量的影响

Figure 2. Effect of different concentrations oleanolic acid on red pea aphid fecundity

下载: 全尺寸图片幻灯片

图 3 BG视讯不同浓度齐墩果酸对绿色型豌豆蚜产蚜率的影响

Figure 3. BG视讯 Effect of different concentrations Oleanolic acid on fecundity rate of green pea aphid

下载: 全尺寸图片幻灯片

图 4 不同浓度齐墩果酸对红色型豌豆蚜产蚜率的影响

Figure 4. BG视讯 Effect of different concentrations oleanolic acid on the fecundity rate of red pea aphid

下载: 全尺寸图片幻灯片

表 1 不同质量浓度齐墩果酸对绿色型豌豆蚜生长发育的影响

Table 1. BG视讯 Effect of the different concentrations oleanolic acid on the growth and development of green pea aphid

齐墩果酸浓度 Oleanolic concentration treatment/(mg·mL^-1)	存活率 Survival rate/%			相对日均体重增长率 Growth rate of relative daily average weight/%	有翅蚜率 Rate of aphid with wings/%
齐墩果酸浓度 Oleanolic concentration treatment/(mg·mL^-1)	第6天 The 6th day	第12天 The 12h day	第20天 The 20th day	相对日均体重增长率 Growth rate of relative daily average weight/%	有翅蚜率 Rate of aphid with wings/%
0 (CK)	96.7 ± 3.3a	60.0 ± 5.8a	33.3 ± 3.3a	0.29 ± 0.01a	3.3 ± 3.3c
0.1 (C₁)	96.7 ± 3.3a	66.6 ± 3.3a	26.7 ± 6.6a	0.26 ± 0.01abc	26.7 ± 3.3b
0.5 (C₂)	96.7 ± 3.3a	66.6 ± 3.3a	20.0 ± 10.0a	0.27 ± 0.01ab	40.0 ± 5.7ab
1.0 (C₃)	96.7 ± 3.3a	53.3 ± 3.3a	20.0 ± 5.8a	0.23 ± 0.01cd	56.7 ± 6.6ab
1.5 (C₄)	93.3 ± 6.6a	56.7 ± 8.8a	0.0 ± 0.0b	0.24 ± 0.01bc	50.0 ± 5.7a
2.0 (C₅)	93.3 ± 3.3a	50.0 ± 5.7a	0.0 ± 0.0b	0.21 ± 0.00d	50.0 ± 11.5a
不同小写字母表示同一指标间不同浓度间差异显著 (P < 0.05)。下同。　Different lowercase letters indicate significant difference between different concentrations of the same index at the 0.05 level; similarly for the following tables and figures.

下载: 导出CSV

表 2 BG视讯不同质量浓度齐墩果酸对红色型豌豆蚜生长发育的影响

Table 2. BG视讯 Effect of different concentrations oleanolic acid on the growth and development of red pea aphid

齐墩果酸浓度 Oleanolic concentration treatment/(mg·mL^-1)	存活率Survival rate/%			相对日均体重增长 Growth rate of relative dailyaverage weight/%	有翅蚜率 Rate of aphid with wings/%
齐墩果酸浓度 Oleanolic concentration treatment/(mg·mL^-1)	第6天 The 6th day	第12天 The 12th day	第20天 The 20th day	相对日均体重增长 Growth rate of relative dailyaverage weight/%	有翅蚜率 Rate of aphid with wings/%
0 (CK)	83.3 ± 6.6a	53.3 ± 3.3a	10.0 ± 0.0a	0.21 ± 0.00a	10.0 ± 0.0c
0.1 (C₁)	83.3 ± 3.3a	46.7 ± 3.3a	6.7 ± 3.3a	0.23 ± 0.01a	20.0 ± 0.0bc
0.5 (C₂)	80.0 ± 0.0a	40.0 ± 10.0a	3.3 ± 0.0a	0.22 ± 0.01a	20.0 ± 5.7bc
1.0 (C₃)	80.0 ± 5.7a	43.3 ± 14.5a	3.3 ± 3.3a	0.22 ± 0.00a	33.3 ± 8.8a
1.5 (C₄)	80.0 ± 5.7a	43.3 ± 14.5a	4.7 ± 3.3a	0.22 ± 0.01a	10.0 ± 5.7c
2.0 (C₅)	73.3 ± 6.6a	40.0 ± 0.0a	3.6 ± 0.0a	0.21 ± 0.00a	13.3 ± 8.8bc

下载: 导出CSV

BG视讯

<i id='b28ad'></i>

点击查看大图

图(4)表(2)

计量

PDF下载量: 6
文章访问数: 146
HTML全文浏览量: 128

通讯作者: 陈斌,

1.
BG视讯shenyanghuagongdaxuecailiaokexueyugongchengxueyuan shenyang 110142

BG视讯

齐墩果酸对豌豆蚜生长发育及繁殖的影响

作者简介: 唐仕娟(1995-)，女，甘肃永登人，在读研究生， 研究方向为植物病理学。E-mail: ;

通讯作者: 王森山, .cn

English

Effect of oleanolic acid on the growth and reproduction of the pea aphid

Corresponding author: Senshan WANG, .cn

BG视讯

计量

文章相关

通讯作者: 陈斌,

作者简介: 唐仕娟(1995-)，女，甘肃永登人，在读研究生，研究方向为植物病理学。E-mail: ;