引用本文: 王思竹，张洵，戴绍军，李莹. 植物ACBP家族成员功能研究进展. 草业科学, 2019, 36(10): 2535-2548. doi: shu

Citation: WANG S Z, ZHANG X, DAI S J, LI Y. Advances in research regarding the function of the ACBP family in plants. Pratacultural Science, 2019, 36(10): 2535-2548. doi: shu

植物ACBP家族成员功能研究进展

.
BG视讯 dongbeiyanjianzhibeihuifuyuzhongjianjiaoyubuzhongdianshiyanshi/dongbeilinyedaxue，dongbeilinyedaxueshengmingkexuexueyuan，heilongjiang haerbin 150040

作者简介: 王思竹(1994-)，女，黑龙江哈尔滨人，在读硕士生，主要从事盐生牧草抗逆分析机理研究。E-mail: ;

通讯作者: 戴绍军, 李莹,

基金项目: 中央高校基本科研业务专项资金(2572018BS03、2572017ET01)

摘要: 酰基辅酶A结合蛋白(Acyl-CoA-binding proteins，ACBPs)是脂类载体蛋白，具有结合和运输脂类物质的功能，在植物生长发育和逆境(低温、干旱和重金属)应答调控过程中发挥了重要作用。本文综述了植物ACBP家族成员的分类、结构特征、生理功能，及其在逆境应答过程中的作用，为深入认识植物ACBP基因家族成员提供了重要信息。

关键词:

English

1. [1]
  HURLOCK A K, ROSTON R L, WANG K, BENNING C. Lipid trafficking in plant cells[J]. TrafficTraffic, 2014, 15(9): 915-932. doi:
2. [2]
  GUIDOTTI A, FORCHETTI C M, CORDA M G, KONKEL D, BENNETT C D, COSTA E. Isolation, characterization, and purification to homogeneity of an endogenous polypeptide with agonistic action on benzodiazepine receptors[J]. Proceedings of the National Academy of Sciences of the United States of America, 1983, 80(11): 3531-3535. doi:
3. [3]
  HILLS M J, DANN R, LYDIATE D, SHARPE A. Molecular cloning of a cDNA from Brassica napus L. for a homologue of acyl-coA-binding protein[J]. Plant Molecular BiologyPlant Molecular Biology, 1994, 25(5): 917-920. doi:
4. [4]
  MENG W, SU Y C, SAUNDERS R M, CHYE M L. The rice acyl-coA-binding protein gene family phylogeny, expression and functional analysis[J]. New PhytologistNew Phytologist, 2011, 189(4): 1170-1184. doi:
5. [5]
  RABOANATAHIRY N, WANG B S, YU L J, LI M T. Functional and structural diversity of acyl-coA binding proteins in oil crops[J]. Frontiers in GeneticsFrontiers in Genetics, 2018, 9(): 182-. doi:
6. [6]
  DU Z Y, ARIAS T, MENG W, CHYE M L. Plant acyl-coA-binding proteins:An emerging family involved in plant development and stress responses[J]. Progress in Lipid ResearchProgress in Lipid Research, 2016, 63(): 165-181. doi:
7. [7]
  NIE Z Y, WANG Y H, WU C T, LI Y, KANG G J, QIN H D, ZENG R Z. Acyl-coA-binding protein family members in laticifers are possibly involved in lipid and latex metabolism of Hevea brasiliensis (the Para rubber tree)[J]. BMC GenomicsBMC Genomics, 2018, 19(1): 5-. doi:
8. [8]
  LEUNG K C, LI H Y, MISHRA G, CHYE M L. ACBP4 and ACBP5, novel Arabidopsis acyl-coA-binding proteins with kelch motifs that bind oleoyl-coA[J]. Plant Molecular BiologyPlant Molecular Biology, 2004, 55(2): 297-309. doi:
9. [9]
  SASAKI Y, NAGANO Y. Plant acetyl-CoA carboxylase:tructure, biosynthesis, regulation, and gene manipulation for plant breeding[J]. Bioscience Biotechnology and Biochemistry, 2004, 68(6): 1175-1184. doi:
10. [10]
  ADAMS J, KELSO R, COOLEY L. The kelch repeat superfamily of proteins: Propellers of cell function[J]. Trends in Cell BiologyTrends in Cell Biology, 2000, 10(1): 17-24. doi:
11. [11]
  GARNIER J, GIBRAT J F, ROBSON B. GOR method for predicting protein secondary structure from amino acid sequence[J]. Methods in EnzymologyMethods in Enzymology, 1996, 266(): 540-553. doi:
12. [12]
  BURTON M, ROSE T M, FAERGEMAN N J, KNUDSEN J. Evolution of the acyl-CoA binding protein (ACBP)[J]. The Biochemical JournalThe Biochemical Journal, 2005, 392(Pt 2): 299-307.
13. [13]
  KELLEY L A, MEZULIS S, YATES C M, WASS M N, STERNBERG M J. The Phyre2 web portal for protein modeling, prediction and analysis[J]. Nature ProtocolsNature Protocols, 2015, 10(6): 845-858. doi:
14. [14]
  COMBET C, BLANCHET C, GEOURJON C, DELEAGE G. NPS@:network protein sequence analysis[J]. Trends in Biochemical SciencesTrends in Biochemical Sciences, 2000, 25(3): 147-150. doi:
15. [15]
  LETUNIC I, DOERKS T, BORK P. SMART: Recent updates, new developments and status in 2015[J]. Nucleic Acids ResearchNucleic Acids Research, 2015, 43(Database issue): 257-260.
16. [16]
  CHYE M L, LI H Y, YUNG M H. Single amino acid substitutions at the acyl-coA-binding domain interrupt 14[C]palmitoyl-coA binding of ACBP2, an Arabidopsis acyl-coA-binding protein with ankyrin repeats[J]. Plant Molecular BiologyPlant Molecular Biology, 2000, 44(6): 711-721. doi:
17. [17]
  LEUNG K C, LI H Y, XIAO S, TSE M H, CHYE M L. Arabidopsis ACBP3 is an extracellularly targeted acyl-coA-binding protein[J]. PlantaPlanta, 2006, 223(5): 871-881. doi:
18. [18]
  GAO W, XIAO S, LI H Y, TSAO S W, CHYE M L. Arabidopsis thaliana acyl-coA-binding protein ACBP2 interacts with heavy-metal-binding farnesylated protein AtFP6[J]. New PhytologistNew Phytologist, 2009, 181(1): 89-102. doi:
19. [19]
  XIAO S, GAO W, CHEN Q F, CHAN S W, ZHENG S X, MA J Y, WANG M F, WELTI R, CHYE M L. Overexpression of Arabidopsis acyl-coA binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence[J]. Plant CellPlant Cell, 2010, 22(5): 1463-1482. doi:
20. [20]
  WANG X. Lipid signaling[J]. Current Opinion in Plant BiologyCurrent Opinion in Plant Biology, 2004, 7(3): 329-336. doi:
21. [21]
  ZHOU Y, ATKINS J B, ROMPANI S B, BANCESCU D L, PETERSEN P H, TANG H, ZOU K, STEWART S B, ZHONG W. The mammalian Golgi regulates numb signaling in asymmetric cell division by releasing ACBD3 during mitosis[J]. CellCell, 2007, 129(1): 163-178. doi:
22. [22]
  WALZ C, GIAVALISCO P, SCHAD M, JUENGER M, KLOSE J, KEHR J. Proteomics of curcurbit phloem exudate reveals a network of defence proteins[J]. PhytochemistryPhytochemistry, 2004, 65(12): 1795-1804. doi:
23. [23]
  YOSHIMOTO K, JIKUMARU Y, KAMIYA Y, KUSANO M, CONSONNI C, PANSTRUGA R, OHSUMI Y, SHIRASU K. Autophagy negatively regulates cell death by controlling NPR1-dependent salicylic acid signaling during senescence and the innate immune response in Arabidopsis[J]. Plant CellPlant Cell, 2009, 21(9): 2914-2927. doi:
24. [24]
  CHEN Q F, XIAO S, QI W, MISHRA G, MA J, WANG M, CHYE M L. The Arabidopsis acbp1acbp2 double mutant lacking acyl-coA-binding proteins ACBP1 and ACBP2 is embryolethal[J]. New PhytolNew Phytol, 2010, 186(4): 843-855. doi:
25. [25]
  XIAO S, GAO W, CHEN Q F, RAMALINGAM S, CHYE M L. Overexpression of membrane-associated acyl-coA-binding protein ACBP1 enhances lead tolerance in Arabidopsis[J]. Plant JournalPlant Journal, 2008, 54(1): 141-151. doi:
26. [26]
  MANJITHAYA R, ANJARD C, LOOMIS W F, SUBRAMANI S. Unconventional secretion of Pichia pastoris Acb1 is dependent on GRASP protein, peroxisomal functions, and autophagosome formation[J]. Journal of Cell BiologyJournal of Cell Biology, 2010, 188(4): 537-546. doi:
27. [27]
  GAO W, LI H Y, XIAO S, CHYE M L. Acyl-coA-binding protein 2 binds lysophospholipase 2 and lysoPC to promote tolerance to cadmium-induced oxidative stress in transgenic Arabidopsis[J]. Plant JournalPlant Journal, 2010, 62(6): 989-1003.
28. [28]
  XIAO S, CHEN Q F, CHYE M L. Light-regulated Arabidopsis ACBP4 and ACBP5 encode cytosolic acyl-coA-binding proteins that bind phosphatidylcholine and oleoyl-coA ester[J]. Plant Physiology and BiochemistryPlant Physiology and Biochemistry, 2009, 47(10): 926-933. doi:
29. [29]
  CHEN Q F, XIAO S, CHYE M L. Overexpression of the Arabidopsis 10-kilodalton acyl-coenzyme A-binding protein ACBP6 enhances freezing tolerance[J]. Plant PhysiollogistPlant Physiollogist, 2008, 148(1): 304-315. doi:
30. [30]
  DU ZY, XIAO S, CHEN Q F, CHYE M L. Depletion of the membrane-associated acyl-coenzyme A-binding protein ACBP1 enhances the ability of cold acclimation in Arabidopsis[J]. Plant PhysiologistPlant Physiologist, 2010, 152(3): 1585-1597. doi:
31. [31]
  LI H Y, CHYE M L. Membrane localization of Arabidopsis acyl-coA binding protein ACBP2[J]. Plant Molecular BiologyPlant Molecular Biology, 2003, 51(4): 483-492. doi:
32. [32]
  LI H Y, CHYE M L. Arabidopsis acyl-coA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats[J]. Plant Molecular BiologyPlant Molecular Biology, 2004, 54(2): 233-243. doi:
33. [33]
  MENG W, HSIAO A S, GAO C, JIANG L, CHYE M L. Subcellular localization of rice acyl-coA-binding proteins (ACBPs) indicates that OsACBP6::GFP is targeted to the peroxisomes[J]. New PhytologistNew Phytologist, 2014, 203(2): 469-482. doi:
34. [34]
  乔坤.极端耐NaHCO₃微藻的鉴定及ACBP抗逆机制研究.哈尔滨: 东北林业大学博士学位论文, 2015.
  QIAO K.Identification of novel highly tolerant NaHCO₃ microalgae and the role for acyl-coA-binding protein (ACBP) under stress tolerance.Harbin: Northeast Forestry University, 2015.
35. [35]
  XIAO S, CHYE M L. An Arabidopsis family of six acyl-coA-binding proteins has three cytosolic members[J]. Plant Physiology and BiochemistryPlant Physiology and Biochemistry, 2009, 47(6): 479-484. doi:
36. [36]
  AZNAR-MORENO JA, VENEGAS-CALERON M, DU Z Y, TANNER J A, CHYE M L, MARTINEZ-FORCE E, SALAS J J. Characterization of a small acyl-coA-binding protein (ACBP) from Helianthus annuus L. and its binding affinities[J]. Plant Physiology and BiochemistryPlant Physiology and Biochemistry, 2016, 102(): 141-150. doi:
37. [37]
  DU Z Y, CHEN M X, CHEN Q F, XIAO S, CHYE M L. Arabidopsis acyl-coA-binding protein ACBP1 participates in the regulation of seed germination and seedling development[J]. Plant JournalPlant Journal, 2013, 74(2): 294-309. doi:
38. [38]
  XUE Y, XIAO S, KIM J, LUNG S C, CHEN L, TANNER J A, SUH M C, CHYE M L. Arabidopsis membrane-associated acyl-coA-binding protein ACBP1 is involved in stem cuticle formation[J]. Journal of Experimental BotanyJournal of Experimental Botany, 2014, 65(18): 5473-5483. doi:
39. [39]
  ZIMMERMANN P, HIRSCH-HOFFMANN M, HENNIG L, GRUISSEM W. GENEVE- STIGATOR. Arabidopsis microarray database and analysis toolbox[J]. Plant PhysiologyPlant Physiology, 2004, 136(1): 2621-2632. doi:
40. [40]
  DU Z Y, CHEN M X, CHEN Q F, XIAO S, CHYE M L. Overexpression of Arabidopsis acyl-coA-binding protein ACBP2 enhances drought tolerance[J]. Plant Cell and EnvironmentPlant Cell and Environment, 2013, 36(2): 300-314. doi:
41. [41]
  ZHENG S X, XIAO S, CHYE M L. The gene encoding Arabidopsis acyl-coA-binding protein 3 is pathogen inducible and subject to circadian regulation[J]. Journal Experimental BotanyJournal Experimental Botany, 2012, 63(8): 2985-3000. doi:
42. [42]
  LI H Y, XIAO S, CHYE M L. Ethylene- and pathogen-inducible Arabidopsis acyl-coA-binding protein 4 interacts with an ethylene-responsive element binding protein[J]. Journal of Experimental BotanyJournal of Experimental Botany, 2008, 59(14): 3997-4006. doi:
43. [43]
  HSIAO A S, YEUNG E C, YE Z W, CHYE M L. The Arabidopsis cytosolic acyl-coA-binding proteins play combinatory roles in pollen development[J]. Plant and Cell PhysiologyPlant and Cell Physiology, 2015, 56(2): 322-333. doi:
44. [44]
  XIAO S, LI H Y, ZHANG J P, CHAN S W, CHYE M L. Arabidopsis acyl-coA-binding proteins ACBP4 and ACBP5 are subcellularly localized to the cytosol and ACBP4 depletion affects membrane lipid composition[J]. Plant Molecular BiologyPlant Molecular Biology, 2008, 68(6): 571-583. doi:
45. [45]
  XIA Y, YU K, GAO Q M, WILSON E V, NAVARRE D, KACHROO P, KACHROO A. Acyl coA binding proteins are required for cuticle formation and plant responses to microbes[J]. Frontiers in Plant ScienceFrontiers in Plant Science, 2012, 3(): 224-.
46. [46]
  JAIN M, NIJHAWAN A, ARORA R, AGARWAL P, RAY S, SHARMA P, KAPOOR S, TYAGI A K, KHURANA J P. F-box proteins in rice.Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress[J]. Plant PhysiologyPlant Physiology, 2007, 143(4): 1467-1483. doi:
47. [47]
  秦朋飞.棉花酰基辅酶A结合蛋白家族基因的发掘及在非生物胁迫抗性中的功能鉴定.南京:南京农业大学硕士学位论文, 2016.
  QIN P F.Genome-wide identification of acyl-coA-binding protein (ACBP) gene family and their functional analysis in abiotic stress resistance in cotton.Nanjing: Nanjing Agricultural University, 2016.
48. [48]
  PASTOR S, SETHUMADHAVAN K, ULLAH A H, GIDDA S, CAO H, MASON C, CHAPITAL D, SCHEFFLER B, MULLEN R, DYER J, SHOCKEY J. Molecular properties of the class Ⅲ subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii)[J]. Plant SciencePlant Science, 2013, 203/204(): 79-88. doi:
49. [49]
  文锦芬, 龚明, 陈凯, 段小凡, 齐亚峰, 王欣欣, 辛艳, 邓明华. 小桐子JcACBP基因克隆和序列分析[J]. 西北植物学报西北植物学报, 2014, 34(11): 2159-2164. doi:
  WEN J F, GONG M, CHEN K, DUAN X F, QI Y F, WANG X X, XIN Y, DENG M H. Cloning and expression analysis of a new acyl-coA-binding protein (JcACBP) identified from Jatropha curcas L[J]. Acta Botanica Boreali-Occidentalia Sinica, 2014, 34(11): 2159-2164. doi:
50. [50]
  NAPIER J A, HASLAM R P. As simple as ACB-new insights into the role of acyl-coA-binding proteins in Arabidopsis[J]. New PhytologistNew Phytologist, 2010, 186(4): 781-783. doi:
51. [51]
  WELTI R, LI W, LI M, SANG Y, BIESIADA H, ZHOU H E, RAJASHEKAR C B, WILLIAMS T D, WANG X. Profiling membrane lipids in plant stress responses.Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis[J]. The Journal of Biological ChemistryThe Journal of Biological Chemistry, 2002, 277(35): 31994-32002. doi:
52. [52]
  DEVAIAH S P, ROTH M R, BAUGHMAN E, LI M, TAMURA P, JEANNOTTE R, WELTI R, WANG X. Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a PHOSPHOLIPASE Dα1 knockout mutant[J]. PhytochemistryPhytochemistry, 2006, 67(17): 1907-1924. doi:
53. [53]
  RADOJKOVIC D, KUSIC J J. Silver staining of denaturing gradient gel electrophoresis gels[J]. Clinical ChemistryClinical Chemistry, 2000, 46(1): 883-884.
54. [54]
  DU Z Y, CHEN M X, CHEN Q F, GU J D, CHYE M L. Expression of Arabidopsis acyl-coA-binding proteins AtACBP1 and AtACBP4 confers Pb(Ⅱ) accumulation in Brassica juncea roots[J]. Plant Cell and EnvironmentPlant Cell and Environment, 2015, 38(1): 101-117. doi:
55. [55]
  SHOYAB M, GENTRY L E, MARQUARDT H, TODARO G J. Isolation and characterization of a putative endogenous benzodiazepineoid (endozepine) from bovine and human brain[J]. The Journal of Biological ChemistryThe Journal of Biological ChemistryBG视讯, 1986, 261(26): 11968-11973.
56. [56]
  SAEZ A, APOSTOLOVA N, GONZALEZ-GUZMAN M, GONZALEZ-GARCIA MP, NICOLAS C, LORENZO O, RODRIGUEZ P L. Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 revealits roleasa negative regulator of abscisic acid signalling[J]. Plant JournalPlant Journal, 2004, 37(3): 354-369. doi:
57. [57]
  KWAK J M, MORI I C, PEI Z M, LEONHARDT N, TORRES M A, DANGL J L, BLOOM R E, BODDE S, JONES J D, SCHROEDER J I. NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis[J]. EMBO JournalEMBO Journal, 2003, 22(11): 2623-1633. doi:
58. [58]
  MICHAELY P, BENNETT V. The ANK repeat:a ubiquitous motif involved in macromolecular recognition[J]. Trends in Cell BiologyTrends in Cell Biology, 1992, 2(5): 127-129. doi:
59. [59]
  LICAUSI F, KOSMACZ M, WEITS D A, GIUNTOLI B, GIORGI F M, VOESENEK L A, PERATA P, VAN DONGEN J T. Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization[J]. NatureNature, 2011, 479(): 419-422. doi:
60. [60]
  GIBBS D J, LEE S C, ISA N M, GRAMUGLIA S, FUKAO T, BASSEL G W, CORREIA C S, CORBINEAU F, THEODOULOU F L, BAILEY-SERRES J, HOLDSWORTH M J. Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants[J]. NatureNature, 2011, 479(): 415-418. doi:
61. [61]
  KOSMACZ M, PARLANTI S, SCHWARZLÄNDER M, KRAGLER F, LICAUSI F, VAN DONGEN J T. The stability and nuclear localization of the transcription factor RAP2.12 are dynamically regulated by oxygen concentration[J]. Plant Cell EnvironmentPlant Cell Environment, 2015, 38(6): 1094-1103. doi:
62. [62]
  XIE L J, YU L J, CHEN Q F, WANG F Z, HUANG L, XIA F N, ZHU T R, WU J X, YIN J, LIAO B, YAO N, SHU W, XIAO S. Arabidopsis acyl-coA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism[J]. Plant JournalPlant Journal, 2015, 81(1): 53-67. doi:
63. [63]
  LIAO P, CHEN Q F, CHYE M L. Transgenic Arabidopsis flowers overexpressing acyl-coA-binding protein ACBP6 are freezing tolerant[J]. Plant and Cell PhysiologyPlant and Cell Physiology, 2014, 55(6): 1055-1071. doi:
64. [64]
  XIAO S, CHYE M L. Overexpression of Arabidopsis ACBP3 enhances NPR1-dependent plant resistance to Pseudomonas syringe pv tomato DC3000[J]. Plant PhysiologistPlant Physiologist, 2011, 156(4): 2069-2081. doi:
65. [65]
  LENZ H D, HALLER E, MELZER E, KOBER K, WURSTER K, STAHL M, BASSHAM D C, VIERSTRA R D, PARKER J E, BAUTOR J, MOLINA A, ESCUDERO V, SHINDO T, VAN DER HOORN R A, GUST A A, NUERNBERGER T. Autophagy differentially controls plant basal immunity to biotrophic and necrotrophic pathogens[J]. Plant JournalPlant Journal, 2011, 66(5): 818-830. doi:
66. [66]
  LIU Y, SCHIFF M, CZYMMEK K, TALLOCZY Z, LEVINE B, DINESH-KUMAR S R. Autophagy regulates programmed cell death during the plant innate immune response[J]. CellCell, 2005, 121(4): 567-577. doi:
67. [67]
  LAI Z, WANG F, ZHENG Z, FAN B, CHEN Z. A critical role of autophagy in plant resistance to necrotrophic fungal pathogens[J]. Plant JournalPlant Journal, 2011, 66(6): 953-968. doi:
1. [1]
  刘博欣 , 赵杨敏 , 庞俊峰 , 孙占敏 , 尉亚辉 , 吴燕民 . 秋茄 ERF 抗逆转录因子的克隆及其功能分析. 草业科学, 2013, 7(11): 1740-1748.
2. [2]
  刘敏国 , 杨倩 , 杨梅 , 杨惠敏 . 藜麦的饲用潜力及适应性. 草业科学, 2017, 11(6): 1264-1271. doi: BG视讯
3. [3]
  徐大伟 , 陈宝瑞 , 辛晓平 . 气候变化对草原影响的评估指标及方法研究进展. 草业科学, 2014, 8(11): 2183-2190. doi:
4. [4]
  陈思慧 , 徐华 , 黄非 , 周功克 , 柴国华 , 董国清 . 草本植物半纤维素研究进展. 草业科学, 2020, 37(3): 506-513. doi: BG视讯
5. [5]
  马清 , 王锁民BG视讯 . 多浆旱生植物霸王质膜Na╋/H╋逆向转运蛋白基因RNAi载体构建. 草业科学, 2012, 6(4): 549-553.
6. [6]
  路子峰 , 张超 , 李彦忠 . 林烟草8个防卫反应信号蛋白编码基因的克隆及RNAi植物表达载体的构建. 草业科学, 2014, 8(7): 1275-1282. doi: BG视讯
7. [7]
  占今舜 , 詹康 , 陈小连 , 霍俊宏 , 赵国琦 . 苜蓿素对脂多糖诱导下奶牛乳腺上皮细胞炎症和乳蛋白合成相关基因表达的影响. 草业科学, 2018, 12(2): 441-448. doi:
8. [8]
  尚明娟 , 曹骏 , 常智慧 . 污泥对草坪草逆境生理的影响. 草业科学, 2017, 11(8): 1591-1600. doi:
9. [9]
  李金博 , 李诗刚 , 徐义炎 , 吴燕民BG视讯 . 白三叶基因工程及逆境生理研究进展. 草业科学, 2013, 7(11): 1842-1851.
10. [10]
  刘文献 , 刘志鹏 , 谢文刚 , 王彦荣 . 脂肪酸及其衍生物对植物逆境胁迫的响应. 草业科学, 2014, 8(8): 1556-1565. doi:
11. [11]
  zhiwuvh+atpmeishiyingnijingdefenzidiaokongjili . caoyekexue, 2013, 7(2): 245-252.
12. [12]
  刘旻霞 , 马建祖 . 种植物在逆境胁迫下脯氨酸的累积特点研究. 草业科学, 2010, 4(4): 134-138.
13. [13]
  产祝龙 , 张慧 , 刘梦垚 . 植物生长调节物质与草坪草及牧草的非生物逆境应答. 草业科学, 2019, 36(12): 3007-3023. doi:
14. [14]
  muxuzonghuangtongduixiaoshuzhileidaixiejiyangziyoujideyingxiang. caoyekexue, 2009, 3(8): 93-96.
15. [15]
  李瑞琴 , 于安芬 , ??蓉 , 徐美蓉 , 宋政平 , 赵有彪 , ??洁BG视讯 . 苜蓿蛋白质分组测定初探. 草业科学, 2009, 3(5): 99-102.
16. [16]
  毛培胜 , 陈泉竹 , 毛培胜 . 种子热激蛋白研究进展. 草业科学, 2016, 10(1): 136-143. doi:
17. [17]
  高丽 , 杨海莉 , 王沛 , 王锁民 . 木栓质及其生理功能. 草业科学, 2018, 12(5): 1218-1231. doi: BG视讯
18. [18]
  BG视讯 caodanbaidoufudeyingyangjiazhiyanjiu. caoyekexue, 2009, 3(8): 47-51.
19. [19]
  BG视讯 jianyehuzhizizhongzizhuzangdanbaifenxi. caoyekexue, 2012, 6(9): 1384-1389.
20. [20]
  惠文森 , 穆晓峰 , 王康英BG视讯 . 草坪草屑叶蛋白质提取方法研究. 草业科学, 2009, 3(3): 108-110.

BG视讯

图 1 拟南芥和水稻ACBPs的结构示意图

Figure 1. Schematic domain structures of the Arabidopsis thaliana and Oryza sativa ACBPs

下载: 全尺寸图片幻灯片

图 2 拟南芥和水稻酰基辅酶A结合蛋白在植物发育各个阶段的表达

Figure 2. BG视讯 The expression of Arabidopsis acyl-CoA-binding proteins at various stages of plant development

下载: 全尺寸图片幻灯片

表 1 BG视讯不同植物中酰基辅酶A结合蛋白(ACBP)家族的组成

Table 1. The acyl-CoA-binding protein (ACBP) family in various plants

植物名称 Plant name	亚家族 Subfamily					总计 Total	参考文献 Reference
植物名称 Plant name	Ⅰ	Ⅱ	Ⅲ	Ⅳ	0	总计 Total	参考文献 Reference
莱茵衣藻 Chlamydomonas reinhardtii	1	0	1	1	0	3	[4]
鞭毛藻 Ostreococcus lucimarinus	1	0	0	1	2	4	[4]
小立碗藓 Physcomitrella patens	2	3	0	2	1	8	[4]
卷柏 Selaginella moellendorffii	1	2	0	1	2	6	[4]
西加云杉 Picea stichensis	1	1	0	1	1	4	[4]
水稻 Oryza sativa	3	1	1	1	0	6	[4]
高粱 Sorghum bicolor	2	1	1	1	0	5	[4]
玉米 Zea mays	2	1	1	1	0	5	[5]
陆地棉 Gossypium hirsutum	2	2	12	8	0	24	[4-5]
拟南芥 Arabidopsis thaliana	1	2	1	2	0	6	[4]
大豆 Glycine max	2	2	4	3	0	11	[5]
蒺藜苜蓿 Medicago truncatula	1	1	0	1	0	3	[4]
蓖麻 Ricinus communis	1	1	1	1	0	4	[5]
可可 Theobroma cacao	1	1	1	1	0	4	[6]
向日葵 Helianthus annuus	2	2	3	3	0	10	[5]
白菜 Brassica rapa	3	2	8	7	0	20	[5]
欧洲油菜 Brassica napus	6	7	16	16	0	45	[5]
甘蓝 Brassica oleracea	3	2	8	7	0	20	[5]
油桐 Vernicia fordii	1	0	1	1	0	3	[5]
木瓜 Carica papaya	1	1	1	1	0	4	[4]
黄瓜 Cucumis sativus	1	1	1	1	0	4	[4]
葡萄 Vitis vinifera	2	1	2	1	0	6	[4]
小桐子 Jatropha curcas	2	1	2	1	0	6	[5]
毛果杨 Populus trichocarpa	2	2	2	1	0	7	[4]
胡杨 Populus euphratica	1	2	2	2	0	7	[4]
油橄榄 Olea europaea	2	2	1	4	0	9	[5]
巴西橡胶树 Hevea brasiliensis	1	1	2	2	0	6	[7]

下载: 导出CSV

表 2 12种植物ACBPs蛋白质模型分类

Table 2. Classification of twelve plants ACBPs protein models

模型名称 Model name	3D 模型 3D structure	结构组成 Structure and composition	植物名称 Plant name
Class Ⅰ
S1		4个α-螺旋；ACB: 3个α螺旋 Four alpha-helices; ACB:three alpha-helices	拟南芥 A. thaliana、欧洲油菜 B. napus、甘蓝 B. oleracea、陆地棉 G. hirsutum
S2		4个紧凑α-螺旋；ACB: 4个α-螺旋 Four closer alpha-helices; ACB:four alpha-helices	白菜 B. rapa、向日葵 H. annuus、小桐子 J. curas、欧洲醡浆草 O. europeae、油桐 V. fordii、玉米 Z. mays
S3		5个紧凑α-螺旋；ACB: 5个α-螺旋 Five closer alpha-helices; ACB:five alpha-helices	水稻 O. sativa
Class Ⅱ
A1		4个α-螺旋及少量β-折叠；ACB: 3个α-螺旋；ANK: 1个α-螺旋 Four alpha-helices and a few beta-sheets; ACB:three alpha-helices; ANK:one alpha-helix	水稻 O. sativa
A2		5个α-螺旋及少量β-折叠；ACB: 4个α-螺旋；ANK: 1个α-螺旋 Five alpha-helices and a few beta-sheets; ACB:four alpha-helices; ANK:one alpha-helix	玉米 Z. mays
A3		5个α-螺旋及少量β-折叠；ACB: 3个α-螺旋；ANK: 1个α-螺旋；Unknown domain: 1个α-螺旋 Five alpha-helices and a few beta-sheets; ACB:three alpha-helices; ANK:one alpha-helix; Unknown domain: one alpha-helix	拟南芥 A. thaliana
A4		6个α-螺旋及少量β-折叠；ACB: 5个α-螺旋；ANK: 1个α-螺旋 Six alpha-helices and a few beta-sheets; ACB:five alpha-helices; ANK:one alpha-helix	白菜 B. rapa、欧洲醡浆草 O. europeae
A5		17个α-螺旋及少量β-折叠；ACB: 9个α-螺旋；ANK: 8个α-螺旋 Seventeen alpha-helices and a few beta-sheets; ACB:nine alpha-helices; ANK:eight alpha-helices	欧洲油菜 B. napus
A6		19个α-螺旋及少量β-折叠；ACB: 4个α-螺旋；ANK: 6个α-螺旋；Unknown domain1: 1个α-螺旋；Unknown domain2: 8个α-螺旋 Nineteen alpha-helices and a few beta-sheets; ACB:four alpha-helices; ANK: six alpha-helices; Unknown domain1: one alpha-helix; Unknown domain1: eight alpha-helices	大豆 G. max
A7		17个α-螺旋及少量β-折叠；ACB: 3个α-螺旋；ANK: 7个α-螺旋；Unknown domain1: 2个α-螺旋；Unknown domain2: 5个α-螺旋Seventeen alpha-helices and a few beta-sheets; ACB:three alpha-helices; ANK:seven alpha-helices; Unknown domain1: two alpha-helices; Unknown domain2: five alpha-helices	小桐子 J. curcas
A8		18个α-螺旋及少量β-折叠；ACB: 3个α-螺旋；ANK: 7个α-螺旋；Unknown domain: 8个α-螺旋 Eighteen alpha-helices and a few beta-sheets; ACB:three alpha-helices; ANK:seven alpha-helices; Unknown domain: eight alpha-helices	陆地棉 G. hirsutum
A9		16个α-螺旋及少量β-折叠；ACB: 4个α-螺旋；ANK: 7个α-螺旋；Unknown domain: 5个α-螺旋 Sixteen alpha-helices and a few beta-sheets; ACB:four alpha-helices; ANK:seven alpha-helices; Unknown domain: five alpha-helixes	甘蓝 B. oleracea、向日葵 H. annuus
Class Ⅲ
L1		4个α-螺旋；ACB: 4个α-螺旋Four alpha-helices; ACB:four alpha-helies	拟南芥 A. thaliana、白菜 B. rapa、欧洲油菜 B. napus、甘蓝 B. oleracea、向日葵 H. annuus、油桐 V. fordii、玉米 Z. mays
L2		4个α-螺旋和部分氨基酸链；ACB: 4个α-螺旋 Four alpha-helices and part of amino acid chain; ACB:four alpha-helices	水稻 O. sativa、陆地棉 G. hirsutum、欧洲醡浆草 O. europeae、小桐子 J. curcas、大豆 G. max
Class Ⅳ
K1		α-螺旋与β-折叠构成3个结构域；ACB: 1个结构域；Kelch: 2个结构域 Alpha-helix and beta-sheet constitute three domains; ACB:one alpha-helix; Kelch:two alpha-helices;	玉米 Z. mays
K2		α-螺旋与β-折叠构成5个结构域；ACB: 1个结构域；Kelch: 3个结构域；Unknown domain: 1个结构域 Alpha-helix and beta-sheet constitute five domains; ACB:one alpha-helix; Kelch:three alpha-helices; Unknown domain: one alpha-helix	大豆 G. max
K3		α-螺旋与β-折叠构成4个结构域；ACB: 1个结构域；Kelch: 3个结构域 Alpha-helix and beta-sheet constitute four domains; ACB:one alpha-helix; Kelch:three alpha-helixes	拟南芥 A. thaliana、欧洲油菜 B. napus、甘蓝 B. oleracea、陆地棉 G. hirsutum、欧洲醡浆草 O. europeae、向日葵 H. annuus、小桐子 J. curcas、水稻 O. sativa、油桐 V. fordii
K4		α-螺旋与β-折叠构成5个结构域；ACB: 1个结构域(无β-折叠)； Kelch: 3个结构域；Unknown domain: 1个结构域 Alpha-helix and beta-sheet constitute five domains; ACB:one alpha-helix(no beta-sheet); Kelch:three alpha-helices; Unknown domain: one alpha-helix	白菜 B. rapa
应用Phyre2软件预测3D模型。粉红色为ACB结构域，A1–A9中蓝色为ANK结构域，灰色表示未知结构域。K1–K4中蓝色、棕色和绿色为kelch结构域，灰色表示未知结构域。　The 3D model was predicted by using the Phyre2 software. Pink indicateds the ACB domain, blue in A1–A9 indicates the ANK domain, and gray indicates the unknown domain. In K11–K4, blue, brown, and green are kelch domains, and gray indicates an unknown domain.

下载: 导出CSV

表 3 拟南芥和水稻ACBPs与磷脂和酰基辅酶A酯的结合特征

Table 3. Binding characteristics of AtACBPs and OsACBPs with phospholipids and acyl-CoA ester

蛋白质 Protein	磷脂结合 Phospholipid binding	酰基辅酶A酯的结合 Acyl-CoA ester binding	参考文献 Reference
Class Ⅰ
AtACBP6	PC(16:0/18:0/18:1/18:2)	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:1-CoA)	[23,25,26,29]
OsACBP1	PA(18:0/18:1) PC(18:0/18:1/18:2)	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:0-CoA) oleoyl-CoA(18:1-CoA) linolenoyl-CoA(18:2-CoA)	[4]
OsACBP2	PA(18:0/18:1) PC(18:0/18:1/18:2)	linoleoyl-CoA(18:2-CoA)	[4]
OsACBP3	PA(18:0/18:1) PC(18:0/18:1/18:2)	linoleoyl-CoA(18:2-CoA)	[4]
Class Ⅱ
AtACBP1	PA(16:0/18:0/18:1) PC(18:1/18:2)	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:1-CoA) linoleoyl-CoA(18:2-CoA) linolenoyl-CoA(18:3-CoA) arachidonyl-CoA(20:4-CoA)	[16-19,24-25,27]
AtACBP2	PC(18:1/18:2) lysolPC	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:1-CoA) linoleoyl-CoA(18:2-CoA) linolenoyl-CoA(18:3-CoA) arachidonyl-CoA(20:4-CoA)	[16-19,24-25,27]
OsACBP4	PA(16:0/18:0/18:1) PC(18:0/18:1/18:2)	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:1-CoA) linoleoyl-CoA(18:2-CoA)	[4]
Class Ⅲ
AtACBP3	PE PC(18:0/18:1/18:2)	linoleoyl-CoA(18:2-CoA) linolenoyl-CoA(18:3-CoA) arachidonyl-CoA(20:4-CoA)	[19,20,24,30]
OsACBP5	PA(18:0/18:1) PC(18:0/18:1/18:2)	palmitoyl-CoA(16:0-CoA) linoleoyl-CoA(18:2-CoA)	[4]
Class Ⅳ
AtACBP4	PC(18:1/18:2)	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:1-CoA) arachidonyl-CoA(20:4-CoA)	[21-24,26,28]
AtACBP5	PC(18:1/18:2)	palmitoyl-CoA(16:0-CoA) oleoyl-CoA(18:1-CoA) arachidonyl-CoA(20:4-CoA)	[21-24,26,28]
OsACBP6	PA(18:0/18:1) PC(18:0/18:1/18:2)	oleoyl-CoA(18:1-CoA) linoleoyl-CoA(18:2-CoA)	[4]
红色字代表强结合性，绿色字代表弱结合性。　Red text indicates strong binding.Green text indicates word weak binding.