引用本文: 丁婷婷，王晓瑜，聂斌，张伟珍，段廷玉. 炭疽病对春箭筈豌豆生长和生理生化的影响. 草业科学, 2019, 36(10): 2569-2579. doi: shu

Citation: DING T T, WANG X Y, NIE B, ZHANG W Z, DUAN T Y. Effect of anthracnose on the growth , physiology, and biochemistry in . Pratacultural Science, 2019, 36(10): 2569-2579. doi: shu

炭疽病对春箭筈豌豆生长和生理生化的影响

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lanzhoudaxuecaodinongyeshengtaixitongguojiazhongdianshiyanshi / lanzhoudaxuenongyenongcunbucaomuyechuangxinzhongdianshiyanshi / lanzhoudaxuecaodinongyekejixueyuan，gansu lanzhou 730020

作者简介: 丁婷婷(1997-)，女，山东东明人，在读硕士生，研究方向为牧草病理学。E-mail: .cn;

通讯作者: 段廷玉, .cn

基金项目:BG视讯国家绿肥产业技术体系(CARS-22)；国家牧草产业技术体系(CARS-34)；教育部创新团队发展计划项目(IRT_17R50)

摘要: 箭筈豌豆(Vicia sativa)是重要的牧草和绿肥，具有较高的经济、生态价值。小扁豆刺盘孢炭疽病(Colletotrichum lentis)是影响箭筈豌豆生长的新病害。本研究通过温室盆栽试验，从植物生长、根瘤形成、病程相关酶以及其他抗病相关物质方面，探究了炭疽病对兰箭3号春箭筈豌豆(Vicia sativa ‘Lanjian No.3’)生长和生理生化的影响。结果表明: 病菌侵染植物后，单独接种病原菌处理与对照相比，两种水解酶活性分别提高33.09%、10.74%，木质素和茉莉酸浓度分别提高26.77%、70.34%。接种根瘤菌的条件下接种病原菌使过氧化物酶等抗病相关酶活性提高36.69%。此外，与对照处理相比病害可导致植物地上、地下生物量降低26.90%～40.00% (P < 0.01)，有效根瘤数降低42.25% (P < 0.05)。因此，炭疽病造成箭筈豌豆抗病相关系统严重受损，从而直接影响到箭筈豌豆的产量及品质，应高度关注该病害的发生，加快防控技术研发。

关键词:

English

Effect of anthracnose on the growth , physiology, and biochemistry in Vicia sativa

state key laboratory of grassland agro-ecosystems / key laboratory of grassland livestock industry innovation, ministry of agriculture and rural affairs/ college of pastoral agricultural science and technology, lanzhou university, lanzhou 730020, gansu, china

Corresponding author: Tingyu DUAN, .cn

Received Date: 2018-06-27
Available Online: 2019-10-01

Abstract: Common vetch (Vicia sativa) is an important pasture and green manure, which has high economic value. Colletotrichum lentis is a new pathogen of common vetch that affect the growth of Vicia sativa. In this study, a pot experiment in greenhouse was established to study the effects of anthracnose on the growth and nodule development of common vetch V. sativa CV, Lanjian No.3 including plant growth, root nodule formation, disease-related enzymes, and other disease-resistant related substances. After the pathogen infected the plants, the two hydrolase activities were increased by 33.09% and 10.74%, respectively, and the concentrations of lignin and jasmonic acid were increased by 26.77% and 70.34%, respectively. In addition, compared with the control treatment, the disease led to a decrease of 26.90%～40.00% (P < 0.01) in the aboveground and underground biomass of plants, and a decrease of 42.25% in the effective nodules (P < 0.05). Therefore, anthracnose causes serious damage to the disease-related system of common vetch, and directly affects the yield and quality of common vetch. Close attention should be paid to the occurrence of this disease and accelerate the development of prevention and control technologies should be accelerated.

Key words:

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图 1 不同处理下有效根瘤数以及植物病情指数

Figure 1. Effective nodule number and disease index for different treatments

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图 2 不同处理下植物地上、地下部分生物量

Figure 2. Shoot biomass and root biomass for different treatments

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图 3 BG视讯不同处理下植物地上、地下部分过氧化物酶活性

Figure 3. Shoot and root peroxidase activity for different treatments

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图 4 BG视讯不同处理下植物地上、地下部分木质素含量

Figure 4. BG视讯 Shoot and root lignin concentration for different treatments

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图 5 不同处理下植物地上、地下部分茉莉酸含量

Figure 5. BG视讯 Shoot and root jasmonic acid concentration for different treatments

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图 6 BG视讯不同处理下植物地上、地下部分多聚半乳糖醛酸酶活性

Figure 6. Shoot and root polygalacturonase activity for different treatments

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图 7 不同处理下植物地上、地下部分果胶甲基酯酶活性

Figure 7. Shoot and root pectinesterase activity for different treatments

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表 1 根瘤菌及小扁豆刺盘孢互作的多因素方差分析P值

Table 1. Rhizobium and Colletotrichum lentis interaction multivariate analysis of variance P value

指标 Parameter	根瘤菌 Rhizobium (R)	小扁豆刺盘孢 Colletotrichum lentis (F)	交互效应 Interactions
指标 Parameter	根瘤菌 Rhizobium (R)	小扁豆刺盘孢 Colletotrichum lentis (F)	R × F
病情指数 Disease index	0.023 0	< 0.000 1	0.023 0
有效根瘤数 Effective number of nodules	< 0.000 1	0.044 5	0.084 2
地上部分生物量 Shoot biomass	0.000 1	< 0.000 1	0.988 9
地下部分生物量 Root biomass	0.000 3	< 0.000 1	0.622 0
地上过氧化物酶 Shoot peroxidase	0.219 3	0.239 2	0.094 3
地下过氧化物酶 Root peroxidase	0.429 4	0.256 1	0.414 7
地上植物木质素 Shoot lignin	0.491 2	0.189 2	0.932 6
地下植物木质素 Root lignin	0.587 3	0.004 1	0.812 4
地上茉莉酸 Shoot jasmonic acid	0.604 9	0.819 7	0.266 4
地下茉莉酸 Root jasmonic acid	0.816 2	0.016 2	0.171 0
地上多聚半乳糖醛酸酶 Shoot polygalacturonase	0.280 6	0.041 3	0.251 3
地下多聚半乳糖醛酸酶 Root polygalacturonase	0.288 9	0.078 6	0.358 5
地上果胶甲基酯酶 Shoot pectinesterase	0.018 8	0.300 0	0.748 9
地下果胶甲基酯酶 Root pectinesterase	0.020 6	0.142 1	0.422 1

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炭疽病对春箭筈豌豆生长和生理生化的影响

作者简介: 丁婷婷(1997-)，女，山东东明人，在读硕士生，研究方向为牧草病理学。E-mail: .cn;

通讯作者: 段廷玉, .cn

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Effect of anthracnose on the growth , physiology, and biochemistry in Vicia sativa

Corresponding author: Tingyu DUAN, .cn

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